You have a syntax error in the configuration file for the entry SimulationStart { Command = ....

GenOpt returns the error message

Command string               : 'cmd /C ""C:\Users\SambuKS\Desktop\EP_Calibration_MinOA\GenOpt\Calib.bat" "Calib" "GBR_Birmingham.035340_IWEC" "C:\EnergyPlusV8-4-0\"  "Measureddata60min_2012.txt""'

which shows that you have only one backslash with quote bracket \" and hence your quotes don't close.